abstract：UNLABELLED:The scorpion family Chaerilidae is phylogenetically differentiated from Buthidae. Their venom components are not known, and the evolution of the venom components is not well understood. Here, we performed a transcriptome analysis of the venom glands from two scorpion species, Chaerilus tricostatus and Chaeri...

journal_title：Journal of proteomics

authors： He Y,Zhao R,Di Z,Li Z,Xu X,Hong W,Wu Y,Zhao H,Li W,Cao Z

更新日期：2013-08-26 00:00:00

abstract：UNLABELLED:Trichoderma reesei is the main industrial producer of lignocellulolytic enzymes, and the secretory behavior of this fungus strongly depends on the carbon sources. To gain insights into how the T. reesei adapts to various carbons and regulates enzyme production, the extra- and intracellular proteomes of T. re...

journal_title：Journal of proteomics

authors： Jun H,Guangye H,Daiwen C

更新日期：2013-08-26 00:00:00

abstract：UNLABELLED:Since the proteins are involved in many physiological processes in the organisms, modifications of proteins have important outcomes. Protein modifications are classified in several ways and oxidative stress related ones take a wide place. Aging is characterized by the accumulation of oxidized proteins and de...

journal_title：Journal of proteomics

authors： Bozaykut P,Sozen E,Kaga E,Ece A,Ozaltin E,Ek B,Ozer NK,Grune T,Bergquist J,Karademir B

更新日期：2013-08-26 00:00:00

abstract：UNLABELLED:Systems biology studies require the capability to quantify with high precision proteins spanning a broad range of abundances across multiple samples. However, the broad range of protein expression in cells often precludes the detection of low-abundance proteins. Different sample processing techniques can be ...

journal_title：Journal of proteomics

authors： Di Girolamo F,Righetti PG,Soste M,Feng Y,Picotti P

更新日期：2013-08-26 00:00:00

abstract：:Proteins in milk have wide range of functions, they are carriers of minerals or chemically vulnerable and insoluble vitamins and other compounds, stabilisers of large aggregates or micelles of lipids, and components of both innate and acquired immune defence systems. Together with other components of milk, proteins ma...

journal_title：Journal of proteomics

authors： Roncada P,Stipetic LH,Bonizzi L,Burchmore RJ,Kennedy MW

更新日期：2013-08-02 00:00:00

abstract：:With the rapid advance of MS-based proteomics one might think that 2D gel-based proteomics is dead. This is far from the truth. Current research has shown that there are still a number of places in the field of protein and molecular biology where 2D gels still play a leading role. The aim of this review is to highligh...

journal_title：Journal of proteomics

authors： Rogowska-Wrzesinska A,Le Bihan MC,Thaysen-Andersen M,Roepstorff P

更新日期：2013-08-02 00:00:00

abstract：UNLABELLED:A proteomics approach based on 2D gel electrophoresis followed by HPLC-electrospray Orbitrap MS/MS was developed to investigate the replacement and the degree of the Se/S substitution in methionine and cysteine in Se-rich yeast. Capillary HPLC-inductively coupled plasma mass spectrometry (ICP-MS), employed i...

journal_title：Journal of proteomics

authors： Bierla K,Bianga J,Ouerdane L,Szpunar J,Yiannikouris A,Lobinski R

更新日期：2013-07-11 00:00:00

abstract：UNLABELLED:The composition and toxicological profile of the venom of the rattlesnake Crotalus simus in Mexico was analyzed at the subspecies and individual levels. Venoms of the subspecies C. s. simus, C. s. culminatus and C. s. tzabcan greatly differ in the expression of the heterodimeric neurotoxin complex 'crotoxin'...

journal_title：Journal of proteomics

authors： Castro EN,Lomonte B,del Carmen Gutiérrez M,Alagón A,Gutiérrez JM

更新日期：2013-07-11 00:00:00

abstract：UNLABELLED:Induced pluripotent stem cells (iPSCs), derived from somatic cells and functionally very similar to embryonic stem cells (ESCs), are at the center stage of intense research in regenerative medicine. We carried out the first membrane proteomic profiling of mouse iPSCs, in comparison with ESCs and adult mouse ...

journal_title：Journal of proteomics

authors： Hao J,Li W,Dan J,Ye X,Wang F,Zeng X,Wang L,Wang H,Cheng Y,Liu L,Shui W

更新日期：2013-06-28 00:00:00

abstract：UNLABELLED:The exoproteome of Leishmania infantum is composed of parasite derived proteins present in the extracellular environment. Although the exoproteome might have a significant role in the precocious steps of infection little is known concerning its composition. We developed an approach enabling the in vitro reco...

journal_title：Journal of proteomics

authors： Santarém N,Racine G,Silvestre R,Cordeiro-da-Silva A,Ouellette M

更新日期：2013-06-12 00:00:00

abstract：UNLABELLED:Cystic hydatid disease is an important zoonosis caused by Echinococcus granulosus infection. The expression profiles of its parasitic life stages and host-Echinococcus interactions remain to be elucidated. Here, we identified 157 adult and 1588 protoscolex proteins (1610 in all), including 1290 novel identif...

journal_title：Journal of proteomics

authors： Cui SJ,Xu LL,Zhang T,Xu M,Yao J,Fang CY,Feng Z,Yang PY,Hu W,Liu F

更新日期：2013-06-12 00:00:00

abstract：UNLABELLED:Fish oil (FO) and tetradecylthioacetic acid (TTA) - a synthetic modified fatty acid have beneficial effects in regulating lipid metabolism. In order to dissect the mechanisms underlying the molecular action of those two fatty acids we have investigated the changes in mitochondrial protein expression in a lon...

journal_title：Journal of proteomics

authors： Wrzesinski K,R León I,Kulej K,Sprenger RR,Bjørndal B,Christensen BJ,Berge RK,Jensen ON,Rogowska-Wrzesinska A

更新日期：2013-06-12 00:00:00

abstract：UNLABELLED:Brevibacterium linens DSM 20158 is an industrially important actinobacterium which is well-known for the production of amino acids and enzymes. However, as this strain has an unsequenced genome, there is no detailed information regarding its proteome although another strain of this microbe, BL2, has a shotgu...

journal_title：Journal of proteomics

authors： Shabbiri K,Botting CH,Adnan A,Fuszard M

更新日期：2013-05-27 00:00:00

abstract：UNLABELLED:In this study, a comparative proteomic analysis was employed to identify fuzz fiber initiation-related proteins in wild-type diploid cotton (Gossypium arboreum L.) and its fuzzless mutant. Temporal changes in global proteomes were examined using 2-DE at five developmental time points for fuzz fiber initiatio...

journal_title：Journal of proteomics

authors： Du SJ,Dong CJ,Zhang B,Lai TF,Du XM,Liu JY

更新日期：2013-04-26 00:00:00

abstract：UNLABELLED:ERLIC and high-pH RP (Hp-RP) have been reported to be promising alternatives to strong cation exchange (SCX) in proteome fractionation. Here we compared the performance of ERLIC, concatenated ERLIC and concatenated Hp-RP in proteome profiling. The protein identification is comparable in these three strategie...

journal_title：Journal of proteomics

authors： Hao P,Ren Y,Dutta B,Sze SK

更新日期：2013-04-26 00:00:00

abstract：UNLABELLED:Fatal events derived from coronary atherosclerosis are the major cause of mortality in the developed countries. Proteomic analysis of the atherosclerotic coronary artery has been mainly carried out with whole tissue extracts, making it difficult to distinguish the alterations present in every region of the p...

journal_title：Journal of proteomics

authors： de la Cuesta F,Zubiri I,Maroto AS,Posada M,Padial LR,Vivanco F,Alvarez-Llamas G,Barderas MG

更新日期：2013-04-26 00:00:00

abstract：UNLABELLED:Sympatric distribution and sharing of hosts and antigens by Trypanosoma rangeli and Trypanosoma cruzi, the etiological agent of Chagas' disease, often incur in misdiagnosis and improper epidemiological inferences. Many secreted and surface proteins (SP) have been described as important antigens shared by the...

journal_title：Journal of proteomics

authors： Wagner G,Eiko Yamanaka L,Moura H,Denardin Lückemeyer D,Schlindwein AD,Hermes Stoco P,Bunselmeyer Ferreira H,Robert Barr J,Steindel M,Grisard EC

更新日期：2013-04-26 00:00:00

abstract：:The experimental isolation and characterization of stable multi-protein complexes are essential to understanding the molecular systems biology of a cell. To this end, we have developed a high-throughput proteomic platform for the systematic identification of native protein complexes based on extensive fractionation of...

journal_title：Journal of proteomics

authors： Wan C,Liu J,Fong V,Lugowski A,Stoilova S,Bethune-Waddell D,Borgeson B,Havugimana PC,Marcotte EM,Emili A

更新日期：2013-04-09 00:00:00

abstract：:Understanding protein interactions within the complexity of a living cell is challenging, but techniques coupling affinity purification and mass spectrometry have enabled important progress to be made in the past 15 years. As identification of protein-protein interactions is becoming easier, the quantification of the ...

journal_title：Journal of proteomics

authors： Tate S,Larsen B,Bonner R,Gingras AC

更新日期：2013-04-09 00:00:00

abstract：:Trypanosoma cruzi proteins with molecular weight between 30 and 34 kDa have shown high reactivity in western blot assays with serum samples from chagasic individuals. However, in-depth analysis of the constituents of these protein fractions has not been performed. This is the first report of an immunoaffinity proteomi...

journal_title：Journal of proteomics

authors： Verissimo da Costa GC,Lery LM,da Silva ML,Moura H,Peralta RH,von Krüger WM,Bisch PM,Barr JR,Peralta JM

更新日期：2013-03-27 00:00:00

abstract：:Neuropeptides are critical signaling molecules, involved in the regulation of diverse physiological processes including energy metabolism, pain perception and brain cognitive state. Prolonged general anesthesia has an impact on many of these processes, but the regulation of peptides by general anesthetics is poorly un...

journal_title：Journal of proteomics

authors： Fouillen L,Petruzziello F,Veit J,Bhattacharyya A,Kretz R,Rainer G,Zhang X

更新日期：2013-03-27 00:00:00

abstract：:High content omic techniques in combination with stable human in vitro cell culture systems have the potential to improve on current pre-clinical safety regimes by providing detailed mechanistic information of altered cellular processes. Here we investigated the added benefit of integrating transcriptomics, proteomics...

journal_title：Journal of proteomics

authors： Wilmes A,Limonciel A,Aschauer L,Moenks K,Bielow C,Leonard MO,Hamon J,Carpi D,Ruzek S,Handler A,Schmal O,Herrgen K,Bellwon P,Burek C,Truisi GL,Hewitt P,Di Consiglio E,Testai E,Blaauboer BJ,Guillou C,Huber CG,Luka

更新日期：2013-02-21 00:00:00

abstract：:MALDI Mass Spectrometry Imaging has shown important potential for molecular classification and pathology marker discovery. Protein markers identification is therefore of prime importance. Direct structural analysis from tissue sections has shown limitations for protein identification because of the high degree of comp...

journal_title：Journal of proteomics

authors： Quanico J,Franck J,Dauly C,Strupat K,Dupuy J,Day R,Salzet M,Fournier I,Wisztorski M

更新日期：2013-02-21 00:00:00

abstract：:Proteomics is becoming more widely used as a tool in fish physiology and toxicology and can offer mechanistic insight into organism responses to environmental signals and stressors. Using a LTQ Orbitrap Velos MS/MS, we detected 1075 proteins in the reproductive testis of fathead minnow. Proteins localized to the testi...

journal_title：Journal of proteomics

authors： Martyniuk CJ,Alvarez S

更新日期：2013-02-21 00:00:00

abstract：:Hyperaccumulators are distinguished from non-hyperaccumulators on the basis of their capacity to extract heavy metal ions from the soil, their more efficient root-to-shoot translocation of these ions and their greater ability to detoxify and sequester heavy metals in the shoot. The understanding of the mechanisms unde...

journal_title：Journal of proteomics

authors： Visioli G,Marmiroli N

更新日期：2013-02-21 00:00:00

abstract：:The majority of common bean plants are cultivated under drought conditions. Maintaining crop yields under drought stress is thus one of the biggest challenges facing bean production. In order to improve our understanding of the complex mechanisms involved in the response of common bean (Phaseolus vulgaris) to drought ...

journal_title：Journal of proteomics

authors： Zadražnik T,Hollung K,Egge-Jacobsen W,Meglič V,Šuštar-Vozlič J

更新日期：2013-01-14 00:00:00

abstract：:Details of metastasis, the deadliest aspect of cancer, are unclear. Cell surface proteins play central roles in adhesive contacts between the tumor cell and the stroma during metastasis. We optimized a fast, small-scale isolation of biotinylated cell surface proteins to reveal novel metastasis-associated players from ...

journal_title：Journal of proteomics

authors： Karhemo PR,Ravela S,Laakso M,Ritamo I,Tatti O,Mäkinen S,Goodison S,Stenman UH,Hölttä E,Hautaniemi S,Valmu L,Lehti K,Laakkonen P

更新日期：2012-12-21 00:00:00

abstract：:Neurodegenerative diseases often lack early and specific diagnostic and prognostic biomarkers. Many studies are focusing on the cerebrospinal fluid (CSF) proteome to identify relevant biomarkers and therapeutic targets for these disorders. An alternative approach consists in comparing proteins secreted by healthy neur...

journal_title：Journal of proteomics

authors： Thouvenot E,Urbach S,Vigy O,Séveno M,Galéotti N,Nguyen G,Bockaert J,Marin P

更新日期：2012-12-21 00:00:00

abstract：:Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen in the world. To identify new vaccine candidates a protein microarray was constructed by expressing the open reading frames (ORFs) from Chlamydia mouse pneumonitis (MoPn). C57BL/6, C3H/HeN and BALB/c mice were immunized either intranasall...

journal_title：Journal of proteomics

authors： Teng A,Cruz-Fisher MI,Cheng C,Pal S,Sun G,Ralli-Jain P,Molina DM,Felgner PL,Liang X,de la Maza LM

更新日期：2012-12-21 00:00:00

abstract：:Celiac disease is an immune-mediated disorder triggered by gluten proteins of wheat (gliadins) and other cereals. Gliadin-mediated effects on weanling animals, sensitized or not with interferon (IFN)-γ, were investigated. Also, the influence of the co-administration of Bifidobacterium longum CECT 7347 was studied toge...

journal_title：Journal of proteomics

authors： Olivares M,Laparra M,Sanz Y

更新日期：2012-12-21 00:00:00

abstract：:The biological variance in protein expression of interest to biologists can only be accessed if the technical variance of the protein quantification method is low compared with the biological variance. Technical variance is dependent on the protocol employed within a quantitative proteomics experiment and accumulated ...

journal_title：Journal of proteomics

authors： Russell MR,Lilley KS

更新日期：2012-12-21 00:00:00

abstract：:The process of protein phosphorylation in cells is well studied in the context of a wide range of biologic functions such as signalling, cell cycle, cell growth and differentiation, and others. In contrast, little progress has been made in the investigation of protein phosphorylation specifically in blood. Here, we fo...

journal_title：Journal of proteomics

authors： Jaros JA,Guest PC,Ramoune H,Rothermundt M,Leweke FM,Martins-de-Souza D,Bahn S

更新日期：2012-12-05 00:00:00

abstract：:Current protocols for quality assurance of platelet concentrates used in transfusion therapy include evaluation of platelet count and pH, in vitro measurements of platelet lysis, membrane activation and microparticle release and assays of platelet ability to respond to aggregation stimuli and to hypotonic shock. Unfor...

journal_title：Journal of proteomics

authors： Rebulla P

更新日期：2012-12-05 00:00:00

abstract：:During blood banking, erythrocytes undergo storage lesions, altering or degrading their metabolism, rheological properties, and protein content. Carbonylation is a hallmark of protein oxidative lesions, thus of red blood cell oxidative stress. In order to improve global erythrocyte protein carbonylation assessment, su...

journal_title：Journal of proteomics

authors： Delobel J,Prudent M,Rubin O,Crettaz D,Tissot JD,Lion N

更新日期：2012-12-05 00:00:00

abstract：:Aggregatability and oxidative damage of red blood cells (RBCs), platelet activation and increased amount of blood cells-derived microparticles (MPs) are thought to be the etiologies for the thrombotic risk in thalassemia, but with unclear mechanisms. Here we report cellular origins and increases in number, oxidative s...

journal_title：Journal of proteomics

authors： Chaichompoo P,Kumya P,Khowawisetsut L,Chiangjong W,Chaiyarit S,Pongsakul N,Sirithanaratanakul N,Fucharoen S,Thongboonkerd V,Pattanapanyasat K

更新日期：2012-12-05 00:00:00

abstract：:Acute myeloid leukemia (AML) is a severe, rapidly progressing disease triggered by blocking granulocyte or monocyte differentiation and maturation. Because of its heterogeneity, AML is divided into a number of subtypes. Unfortunately, so far very few correlations have been found between AML classification and its clin...

journal_title：Journal of proteomics

authors： Luczak M,Kaźmierczak M,Handschuh L,Lewandowski K,Komarnicki M,Figlerowicz M

更新日期：2012-10-22 00:00:00

abstract：:Cold storage is being used to increase nectarine fruits' postharvest life. However, low temperatures lead to chilling injury and limit their commercial quality and value. In this study a proteomic approach was used to compare the protein profile between control and cold storage nectarine fruits. Protein extracted from...

journal_title：Journal of proteomics

authors： Giraldo E,Díaz A,Corral JM,García A

更新日期：2012-10-22 00:00:00

abstract：:The present study aimed the evaluation of saliva sample pre-treatment, in particular the sample clearance usually performed by centrifugation, to the contribution of salivary proteome and peptidome. Using in-gel and off-gel approaches, a large content of salivary proteins was detected in the pellet fraction that is us...

journal_title：Journal of proteomics

authors： Vitorino R,Guedes S,Manadas B,Ferreira R,Amado F

更新日期：2012-09-18 00:00:00

abstract：:In the development of ischemia/reperfusion (I/R) injury, the role of the myosin light chain (MLC) phosphorylation has been given increased consideration. ML-7, a MLC kinase inhibitor, has been shown to protect cardiac function from I/R, however the exact mechanism remains unclear. Isolated rat hearts were perfused und...

journal_title：Journal of proteomics

authors： Lin HB,Cadete VJ,Sawicka J,Wozniak M,Sawicki G

更新日期：2012-09-18 00:00:00

abstract：INTRODUCTION:Juvenile idiopathic arthritis (JIA) comprises a poorly understood group of chronic autoimmune diseases with variable clinical outcomes. We investigated whether the synovial fluid (SF) proteome could distinguish a subset of patients in whom disease extends to affect a large number of joints. METHODS:SF sam...

journal_title：Journal of proteomics

authors： Gibson DS,Newell K,Evans AN,Finnegan S,Manning G,Scaife C,McAllister C,Pennington SR,Duncan MW,Moore TL,Rooney ME

更新日期：2012-09-18 00:00:00